In recent years, CRISPR/Cas technology has become an indispensable tool for genome editing. CRISPR technology goes beyond gene knockout and can also affect how a gene functions using CRISPR activation or CRISPR interference (CRISPRa or CRISPRi) technology.
Cellecta is proud to offer a complete solution for gene activation and gene repression screening with pre-made and custom pooled lentiviral libraries for both CRISPRa targeted gene activation and CRISPRi targeted gene repression screens. These modified CRISPR platforms provide alternative genetic screening approaches that enable the identification of genes whose modulation--either repression or activation--initiates a response.
Cellecta is proud to offer a complete solution for gene activation and gene repression screening with pre-made and custom pooled lentiviral libraries for both CRISPRa targeted gene activation and CRISPRi targeted gene repression screens. These modified CRISPR platforms provide alternative genetic screening approaches that enable the identification of genes whose modulation--either repression or activation--initiates a response.
CRISPRa and CRISPRi Libraries for Screening
CRISPRa targeted gene activation and CRISPRi targeted gene repression systems provide alternative genetic screening approaches to identify genes required to maintain a biological response. To simplify your workflow, Cellecta offers off-the-shelf and custom lentiviral libraries
CRISPRa targeted gene activation and CRISPRi targeted gene repression systems provide alternative genetic screening approaches to identify genes required to maintain a biological response. To simplify your workflow, Cellecta offers off-the-shelf and custom lentiviral libraries
- genome-wide, single-guide CRISPRa and CRISPRi libraries for human and mouse systems
- genome-wide, dual-guide libraries which comprise two sgRNAs targeting each gene for stronger activation and repression (dual-sgRNA constructs library)
- both plasmid (200 ug) and pre-packaged lentiviral particle (2x 10^8 TU & 1x 10^9 TU) formats
- custom sgRNA libraries for any set of genes you want to target, with a wide variety of vectors, selection markers, etc.
CRISPRa
CRISPRi
How it works
The Cas9 protein is engineered to eliminate nuclease activity and produce a deactivated Cas9 (dCas9) that binds tightly to the sgRNA so it can be targeted to essentially any location in the genome. When the dCas9 protein is complexed with a gene repressor protein (i.e., the KRAB protein), it forms a complex that can inhibit gene expression (CRISPRi) when targeted to the upstream regulatory region of a target gene.
Conversely, gene transcription activators (e.g., VPH) can be linked to the sgRNA/dCas9 to make a complex that can be used to activate a gene of choice (CRISPRa).
Since the effect does not alter the underlying DNA sequence, this activation or repression is reversible.
Conversely, gene transcription activators (e.g., VPH) can be linked to the sgRNA/dCas9 to make a complex that can be used to activate a gene of choice (CRISPRa).
Since the effect does not alter the underlying DNA sequence, this activation or repression is reversible.
Some applications
1. Assess the effects of activation or repression of thousands of different genes on cell growth or other screenable phenotypes using pools of sgRNA constructs that target the promoter or transcript start regions of genes.
2. Identify potential therapeutic targets responsible for controlling growth and differentiation, regulating disease development, or other biological responses.
3. Dual-sgRNA CRISPRa and CRISPRi libraries extend the range of tools available for more efficient functional genetic screening and accelerate the identification of novel targets for therapeutics and biomarker analysis.
2. Identify potential therapeutic targets responsible for controlling growth and differentiation, regulating disease development, or other biological responses.
3. Dual-sgRNA CRISPRa and CRISPRi libraries extend the range of tools available for more efficient functional genetic screening and accelerate the identification of novel targets for therapeutics and biomarker analysis.
Cellecta also provides CRISPRa and CRISPRi Constructs downstream validation and other applications
For more targeted or specialized sets of genes, Cellecta can build custom libraries and constructs to suit your requirements.
Contact us to learn how our expert team with over 15 years of genetic screening and library construction experience can help your screening experiment succeed.
Contact us to learn how our expert team with over 15 years of genetic screening and library construction experience can help your screening experiment succeed.
Cellecta has proven expertise and extensive capabilities to generate high-quality, complex CRISPR sgRNA libraries targeting virtually any defined sequences, incorporating a range of features for CRISPRa and CRISPRi.
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- Custom sgRNA libraries for CRISPRa, and CRISPRi target your genes of interest
- Incorporate any desired guide and tracrRNA variations, e.g. HEAT tracr, 10X feature barcodes, dCas9-SAM tracr, etc.
- Various library formats offered, including plasmid only and pre-packaged, ready-to-transduce lentiviral particles.
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Interference or activation of your target gene with custom CRISPRi, or CRISPRa sgRNA lentiviral constructs.
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- Flexible design services to ensure the sgRNA targets the genes, isoforms, or regulatory elements you want.
- A range of vector designs, including sgRNA-only, sgRNA-Cas9, dual-sgRNA, inducible sgRNA vectors.
- Non-targeting, non-specific, and other control guides available in any vector backbone.
- Lentiviral plasmids and pre-packaged lentiviral particle formats available.
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To learn more about these and other Cellecta products and custom services, visit www.cellecta.com
or email us at info@cellecta.com
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or email us at info@cellecta.com
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